久久久天堂国产精品女人,无码午夜福利片,天天摸夜夜摸夜夜狠狠摸,欧美亚洲精品一区二三区

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2549Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

簡(jiǎn)要描述:Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2549
  • 廠(chǎng)商性質(zhì):生產(chǎn)廠(chǎng)家
  • 更新時(shí)間:2025-04-28
  • 訪(fǎng)  問(wèn)  量:1447

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2549™    Price:$417.00
Designations:Panc 03.27Depositors:EM JaffeeBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞Tumorigenic:YesOncogene:K-ras + (wild-type)Antigen Expression:MHC class I +; MHC class II - [50655] Blood type A; Rh+DNA Profile (STR):Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 12 D5S818: 12,13 D7S820: 8,13 THO1: 6 TPOX: 8,9 vWA: 16,17Age:65 yearsGender:femaleEthnicity:WhiteComments:Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                               Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseDoubling Time:37.5 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-20200.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101Cell culture tested DMSO:ATCC 4-XReferences:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲av不卡无码国产| 东北老女人高潮大喊舒服死了| 亚洲欧洲自拍拍偷综合| 国产熟妇勾子乱视频| 一区二区视频在线播放| 国产九九99久久99大香伊| 国产成人免费高清激情视频| 深夜放纵内射少妇| 久久国产精品久久精品国产| 精品一区二区三区无码中文视频| 人妻va精品va欧美va| 国产精品无卡毛片视频| 日本亚洲中文字幕不卡| 亚洲国产午夜精品理论片妓女| 日产一二三区别免费必看| 一本色道久久88—综合亚| 国产精品民宅偷窥盗摄| 亚洲精品一区二区18| 欧美一区二区三区四区五区| 国产精品嫩草影院av蜜臀| 日本久久一区二区| 嫩草精品| 人妻少妇被粗大爽| 少妇熟女av| 天堂资源最新在线| 久久成人18免费网站| 伊人久久大香线蕉av影院| 性欧美18一69性sexhd| 哈巴河县| 四虎永久在线精品国产馆V视影院| 遂平县| 亚洲国产欧美在线人成aaaa| 啊灬啊灬啊快日出水了| 久久精品无码午夜福利理论片| 337P日本大胆欧美裸体艺术| 亚欧成人无码AV在线播放| 亚洲午夜成人精品无码| 久久婷婷五月综合色国产免费观看 | 伊人久久综在合线亚洲2019| 无码av动漫精品一区二区免费| 久久午夜夜伦鲁鲁片免费无码|