久久久天堂国产精品女人,无码午夜福利片,天天摸夜夜摸夜夜狠狠摸,欧美亚洲精品一区二三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CCL-220™COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

簡要描述:COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CCL-220™
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:1562

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

COLO 320DM (ATCC® CCL-220™)

Organism  Homo sapiens, human

Tissue  colon

Product Format  frozen

Morphology  rounded and refractile

Culture Properties  mixed, adherent and suspension

Biosafety Level  1

Disease  Dukes' type C, colorectal adenocarcinoma

Age  55 years

Gender  female

Ethnicity  Caucasian

Storage Conditions

Karyotype  modal number = 24; range = 49 to 61.

The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.

Images

Clinical Data  55 years

Caucasian

female

Genes Expressed  serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone

Tumorigenic  Yes

Effects  Yes, in nude mice

Comments  The line is negative for CEA, CSAp and colon antigen 3.

The cells are weakly positive for keratins and vimentin

Complete Growth Medium  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

Subculturing  Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.

1.Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.

2.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

3.Add appropriate aliquots of the cell suspension to new culture vessels.

4.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation  Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: Liquid nitrogen vapor phase

Culture Conditions  Temperature: 37°C

STR Profile  Amelogenin: X

CSF1PO: 11

D13S317: 11

D16S539: 11,12

D5S818: 12

D7S820: 9,12

THO1: 9

TPOX: 8,9

vWA: 15,18

Isoenzymes  ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Name of Depositor  GE Moore

References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
噼里啪啦中文免费观看高清手机看 | 欧美在线综合| 韩国精品一区二区三区四区| 久久久久久久波多野结衣高潮| 国产激情视频一区二区三区| 亚洲AV成人中文无码专区| 金湖县| 亚洲乱妇老熟女爽到高潮的片| 37p粉嫩大胆色噜噜噜| 色综合小说| 亚洲综合色无码| 中文无码热在线视频| 无码人妻一区二区三区免费看| 少妇XXXXX性开放| 欧美日韩人妻精品一区二区三区| 国产精品久久久区三区天天噜| 老师和大学生一级一片| 丝袜亚洲精品中文字幕| 天天综合网亚在线| 自拍偷在线精品自拍偷99| 国产精品VIDEOSSEX久久发布| 一本一本久久A久久综合精品| 亚洲欧美综合精品成人导航| 韩城市| 国产乱码一区二区三区| 日韩第一页| 国产精品久久久久久久久久尿| 国产性生活| 欧美另类xxxx| 九九99九九精彩46| 欧洲精品久久久久69精品| 久久国产精品一区二区| 国产精品久久久久久网站| 亚洲大片69999| www黄片com| 91精品夜夜夜一区二区| 水蜜桃精品一区二区三区| 日本美女一区二区| 天堂av亚洲av一二三区| 中文字幕一区二区人妻痴汉电车 | 性欧美video另类hd野外|