久久久天堂国产精品女人,无码午夜福利片,天天摸夜夜摸夜夜狠狠摸,欧美亚洲精品一区二三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-11268 293T/17人胚腎細(xì)胞

CRL-11268 293T/17人胚腎細(xì)胞

簡要描述:CRL-11268 293T/17人胚腎細(xì)胞
,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1621

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CRL-11268 293T/17人胚腎細(xì)胞

Permits and Restrictions

View Restrictions

OrganismHomo sapiens, human
Tissuekidney
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level2 [Cells contain Adeno and SV-40 viral DNA sequences]
Agefetus
Applications

These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.  CRL-11268 293T/17人胚腎細(xì)胞

Storage Conditionsliquid nitrogen vapor phase
Derivation

The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a hmperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned anighly transfectable derivative of the 293 cell line into which the ted the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17.

Antigen Expression

SV40 T antigen

Genes Expressed

SV40 T antigen

Comments

293T/17 cells were cotransfected with the pCRIPenv- and the pCRIPgag-2 vectors to obtain the ANJOU 65 (see ATCC CRL-11269) cell line. ANJOU 65 cells were cotransfected with the pCRIPgag-2 and pGPT2E vectors to obtain the BOSC 23 (see ATCC CRL-11270) ecotropic envelope-expression packaging cell line. ANJOU 65 cells were also cotransfected with the pCRIPAMgag vector along with a plasmid expressing the gpt resistance gene to obtain the Bing (see ATCC CRL-11554)amphotropic envelope-expression packaging cell line.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.      
SubculturingVolumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

S

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
好爽好紧好大的免费视频国产 | 中文字幕无码日韩AV| 国产午夜片无码区在线观看| 狼友网精品视频在线观看| 欧美激情综合色综合啪啪五月| 久久久久久久av| 囊谦县| 国产一区二区三区精品视频 | www一区二区三区| 蜜桃av在线播放| 久久a| 欧美人妻久久精品二区三区特黄| 香蕉夜夜草草久久亚洲香蕉| 精品内射| 亚洲av永久无码老湿机漫画| 亚洲日韩国产欧美一区二区三区| 成人免费一区二区三区视频| 国产高清不卡一区二区| 亚洲欧美成人精品香蕉网| 色诱久久av| 夜夜揉揉日日人人青青| 国产av综合第一页| 狼狼综合久久久久综合网| 亚州欧美| 午夜www| 精品人人妻人人澡人人爽牛牛| 少妇精品| 亚洲日韩亚洲另类| 五月丁香六月综合AV| 同性男男黄G片免费网站| 在线岛国片免费无码AV | 99久久无色码中文字幕| 精品国产乱码久久久久久蜜桃网站 | 超碰人人澡| 水城县| 999AV| 超碰色偷偷男人的天堂| 8AV国产精品爽爽ⅴa在线观看| 人妻va精品va欧美va| 成人片国产精品亚洲| 色呦呦在线|