久久久天堂国产精品女人,无码午夜福利片,天天摸夜夜摸夜夜狠狠摸,欧美亚洲精品一区二三区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1739AGS 人胃腺癌細(xì)胞

AGS 人胃腺癌細(xì)胞

簡要描述:CRL-1739 AGS 人胃腺癌細(xì)胞
上海復(fù)祥生物公司現(xiàn)貨供應(yīng)美國典型培養(yǎng)物保藏中心(ATCC)各類細(xì)胞,*。 手機(jī) xiangfbio.歡迎各位老師來電咨!

  • 產(chǎn)品型號(hào):CRL-1739
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:2341

產(chǎn)品分類

Product Category

詳細(xì)介紹

RL-1739 AGS 人胃腺癌細(xì)胞 的詳細(xì)介紹

AGS 人胃腺癌細(xì)胞

ATCC® Number:  CRL-1739™

Designations:  AGS

Depositors:   SC Barranco

Biosafety Level: 2

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: stomach

Disease: gastric adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation:  Isolation date: 1979

Applications: transfection host (Nucleofection technology from Lonza)

Tumorigenic: Yes

Cytogenetic Analysis: This is a hyperdiploid human cell line. The modal chromosome number was 49, occurring in 60% of cells. The rate of polyploidy was 3.6%. Single copy each for der(8)t(1;8) (q12;p23), der(19)t(19;?) (q13.6;?), minute chromosome M3, and C-group-like M12 was seen in all cells. The origins of both M3 and M12 defied identification presently. The t(13q14q) occurred in some. Generally there were three copies for N20, and single copy for X, N8 and N18. Occasionally there were three copies for N14.

Age:  54 years

Gender:  female


Ethnicity:  Caucasian

Comments: The AGS cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy. The cells have a plating efficiency of 34% in the medium below. The line was cured at the ATCC of a prior mycoplasma infection .Subsequently, AGS has been determined to be infected with Parainfluenza type 5 (PIV5 formerly known as SV5). [PubMed: 17509637]

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time:  20 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 22748: Barranco





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
男男gv无码免费无禁网站| www综合五月婷| 亚洲国产精品日韩av不卡在线 | 国产精品成人一区二区三区 | 超碰97在线免费| 日韩欧美区| 国产精品久久久久久久久久久 | 先锋影音最新色资源站| 久久国产精品99精品国产| 精品国产乱码久久久久久小说| 欧美aaa| 第一章少妇初尝云雨69章| 男男搞基网站| 亚洲国产精品一区二区尤物区| 欧美午夜理伦三级在线观看| 双乳奶水饱满少妇呻吟| A级国产乱理论片在线观看| A级毛片100部免费看| 故城县| 香河县| 一区二区三国产精华液| 天天日天天干天天操| 亚洲综合激情网| 夜夜躁狠狠躁| 午夜精品久久久内射近拍高清| 亚洲日产一线二线三线精华液 | 无码无遮挡又大又爽又黄的视频| 无码aⅴ在线观看| 丰满熟妇乱又伦精品| 成在人线av无码免费看| 丰满熟女人妻一区二区三| 色呦色呦色精品| 超碰人人插| 成人777| 蜜臀久久精品久久久久| 人与物videos另类| 人妻的秘密| 天天爽夜夜爽夜夜爽精品视频| 日韩日韩日韩日韩日韩日韩日韩| 婷婷精品视频| 久久国产一区二区三区|